Recombinant DNA technology, also called molecular cloning, involves the replication of DNA in a living microorganism
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Recombinant DNA technology, also called molecular cloning, involves the replication of DNA in a living microorganism. The cloning of any DNA fragment involves seven steps (Boundless, 2016):
- Choice of host organism and cloning vector
- Preparation of vector DNA
- Preparation of DNA to be cloned
- Creation of recombinant DNA
- Introduction of recombinant DNA into host organism
- Selection of organisms containing recombinant DNA
- Screening for clones with desired DNA inserts and biological properties
According to the Universal Genetics DNA Testing Laboratory, one of the current technologies used for paternity testing is Polymerase Chain Reaction (PCR). PCR is used to amplify a single copy of a piece of DNA by in vitro enzymatic replication in order to generate many copies of the DNA sequence. The steps of PCR include (United Genetics, 2009):
- Initialization
- Denaturation
- Annealing
- Extension/elongation
- Final elongation
- Final hold
One other technology utilized for DNA paternity testing is Restriction Fragment Length Polymorphism (RFLP) which actually includes PCR. The steps of RFLP include (Thermo Fisher Scientific, n.d.):
- Isolate DNA
- Perform PCR
- Perform restriction digestion
- Prepare sample for analysis
- Perform capillary electrophoresis
- Analyze data
– Melissa
References
Boundless. (2016, August 8). Recombinant DNA technology. Retrieved from
https://www.boundless.com/microbiology/textbooks/boundless-microbiology-textbook/microbial-genetics-7/tools-of-genetic-engineering-82/recombinant-dna-technology-447-5735/
Thermo Fisher Scientific. (n.d.). Restriction fragment length polymorphisms (RFLP) analysis. Retrieved from
https://www.thermofisher.com/us/en/home/life-science/sequencing/fragment-analysis/restriction-fragment-length-polymorphism-rflp-analysis.html
United Genetics. (2009). Polymerase chain reaction (PCR). Retrieved from
http://www.dnatestingforpaternity.com/pcr.html